20 Bullclzbz N0. 98.  
was demonstrated in our plots in 1901 that the tubercles can ·
be grown in great numbers on the roots of soy beans by the use .
of pure cultures of the tubercle bacteria. Through the kind-
ness of Dr. L. Hiltner, of Berlin, Germany, Ireceived in june,
1901, test tubes containing cultures of bacteria for soy beans,
red clover, and alfalfa. It was too late when they arrived to ·
get fair tests of the cultures on red clover and alfalfa, and
the attempts failed. On the soy bean the time appeared to be
most opportune. The growth in one of the tubes was turned
into a small beaker containing enough distilled water to
moisten thoroughly seeds sufficient to plant I—8O acre, The
gelatine bearing the growth was broken up so as to mix the
bacteria with the water. After sprinkling the soy beans with _
this, they were mixed with sand and set away in a dark cup-
board until ready to plant. The sand and seeds together were_
then sown in drills, and beside the plot another 1-80 acre was
sown with untreated seed. Both lots grew off well, with no
apparent difference above ground in the plants of the two `
plots. But when about eight inches high, tubercles began to
appear on the roots of the treated lot and a little later they
became thickly covered. Probably not a plant was entirely
free from them. The plants in the adjacent check lot pro-
duced no tubercles during the season.
The experiment was a complete success, and if I may
judge by these results, the cultures for clover and alfalfa
would also have succeeded if tried at the proper season for
sowing these plants. I wish to acknowledge here the kind-
ness of Dr. Hiltner, and to thank him for this opportunity to
test the cultures.
Iyzseci and 0{her wzevrzzks. At present the plants are
attacked by but few insects, and appear to be equally free
from fungus enemies. Grasshoppers (mostly the red-legged
grasshopper) gnawed the leaves during a dry period last
summer, and a reddish brown hairy caterpillar (Spz`Z0s0¢zza
z·z`z;g·z‘zzzkrz) at one time ate numerous holes inthe leaves of
both soy beans and cowpeas, but was destroyed later by a _ 
parasitic fungus (Empusa) that left the dried bodies in large
numbers sticking to the leaves. A few leaf-mines were ob-