1
l
I
l
l
_   Ifeatue/ty Agricultural Expertiutexzt Station. 65
  slightly nitrified and the azid scarcely at all. The interesting
  observation was made that where ammonia salt and cyanide were 1
l used together, the nitritication seemed to be arrested at the nitrite
Q stage, as shown b_y a considerable production of nitrite, with
  relatively little nitrate.
l During the spring of 1916, white clover was unusually plen-
  tiful in the pastures and bloating was very prevalent among
  cows and cattle. This atfordcd a good opportunity to further
  test the formaldehyde treatment proposed by this department. ‘
  In a large majority of cases, both coming under our observation
le   and reported by others, the treatment resulted in a cure. The suc- .
{_   cess of our method of treatment 1nay be considered as established.
in   This spring cows and cattle bloated badly on fresh clover, be-
l)' i fore blossoming time, a new experience here. This led us to '
le- Q examine the distribution of sugar in the clover plant, both be-
g-   fore and after blossoming. lt was found that small, immature
ly , _ plants, before blossoming, contained 1.2 per cent. of sugar; the i
in- l leaves and stems of blossoming plants contained 1.43 per cent.
rt- and the blossoms contained 1.55 per cent. Former tests had
shown that bluegrass, leaves of clover and alfalfa contained
UQ less than 1 per cent of sugar. It thus appears that. in seasons of l I
S0- unusually good growth of clover, there is an increased production
re- of sugar and mueh of it may be stored in the plant. before blos-
ng soming time. \Ve further demonstrated the presence on fresh
ml: clover plants of several species of bacteria capable of fermeuting V i
ere sugar, with formation of carbon dioxid.
lu` Much work was done on the isolation and identification
lfm of hog cholera virus. Having previously succeeded in isolating
ml _ a tilterable organism. as described in a paper under this title
thc lll the Journal of lnfectious Diseases. .lanuar_v. ltllti. we llave
mu fllrther succeeded in obtaining this organism from the virus blood
TL $Cl'll11l. growing it. in a special medium which eontained no hog
for tissue other than the small quantity of virus serum added. lix-
periments i11 inoculating normal shotes with these cultures seem
The lt) show that hog cholera was coniniunieated to ll(‘illtll}' Shot<‘S lll
mm llll$ Way. VVe desire to verify this observation, however, by YC-
mg peating these experiments.
»lll}'
I`