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23 OHEMISTRV O MOLECULAR BIOLOGY
8 AM HEBlSTRATlUN 8 CUNHNENTAL BREAKFAST internal molecular scaffolds, which also orient most of the cells D J 00:)
Gallery, WT Young Library biochemical processing machinery. This structural perspective
has led to new insights into the molecular basis of cellular . 1/
mechanotransduction  the process by which living cells sense , I" /,
8 AB mechanical forces and convert them into changes in intracellular . _, 4/ . . .
i AM WELCOME biochemistry It also has led to the creation of human "organ~on .  . ' .
Dr. Eli Capilouto, University of Kentucky President aachip" microdevices that recapitulate the complex structures and . . .
Auditorium, W.T. Young Library functions of living organs, which represent powerful new in vitro  . I. '. \ , ,1 ,
tools for modeling human physiology and disease. EST, l: I M ED Ellllllwlg ,, NTUN NAEF .
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Arizona State University lligm AM LUNCl-l o 3'3'12253 any;  '
Designer Architectures for Programmable Selfassemb/y : ...,-" , \,, . .512:fo . . .
The centraltask of nanotechnology is to control motions and l'gm CUMDLEXITV AND SELF ASSEMBLY
organize matter with nanometer precision To achieve this, ' DM DUSTEH SESSlUN ,. ., , , .. .
scientists have investigated a large variety of materials including Ballroom, ng Alumni House ' vflt - . . W .,
inorganic materials, organic molecules, and biological polymers , 7 9V (l ll "(,3 i[ w l
as well as different methods that can be sorted into so-called litllll a A, APR )9 'zl _,. . @5133 , 
"bottomup" and "topdown" approaches, Among all of the 2.3% pM DR TODD VEATES " ' _ 'T l g,
' remarkable achievements made, the success of DNA self '. . '. . "1::7

. . . Univer5ity of California Los Angeles .2 ,
assembly in bUIlding programmable nanopatterns has attracted Gi nt Protein Ca es and Assemblies in Nature and b Desi n l 7,
broad attention. in this talk I will present our efforts in using DNA a g y g D llplE/Alyqumn .'

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as an information coding polymer to program and construct Nature has evolved myriad sophisticated structures based Lu 9) :11 l 51] '3 ",1 Ma
DNA nanoarchitectures With complex geometrical features Use . . I ; ,,,_v,;,,;_cg,;;3;.{z;gt

. . on the assembly of protein subunits Many types of natural 7 7 l , i , ,.,;i,,7,,_;i-,;tyl.yv,45,333
of deSIgner DNA architectures as molecular sensor, actuator and . . , . . U) m I] Ill l [l \\ (1 \ if}:"ll"li'l,,47:725il1}

r . protein assemblies (such as Virus capSIds) have been studied l], p ,l l {,1} i3

scaffolds Will also be discussed. . . . , L0 LIJ  7:7,:5,7E,~,_,7,.z~
extenswely, while a number of equally sophisticated natural > LO 3 , 7 7 1,. _ . ~ 4;,

protein assemblies are only beginning to be appreciated Among h 0 0 ll DUN/M l1 lllflllwgl , 

1m AM 8 the latter group is a broad class of giant, capsidlike assemblies *5; > Cl) LIJ r . , w 1i _. I i. . u an if

BREAK REFRESHMENTS referred to as bacterial microcompartments. They serve as "' x to D: 7, - .7 ,, , A N f

primitive metabolic organelles in many bacteria by encapsulating g g o LLI lUlBL llllllllllll \VAE/Alllgy :

sequentially acting enzymes within a selectively permeable .C 4.. L0 U
'lm 3% protein shell. Our laboratory has elucidated key mechanisms 0 C O S

i AM DR. DONALD E. lNGBER ofthese protein-based bacterial organelles through structural i... g V CK , l, , , V ,, , I p, m" 7V

Harvard University studies. On the engineering side, sophisticated natural protein 0 _ >- DJ ngllllllllllglllll 1m: iLlllglllll'ylmM
From Cellular Mechanotransduction to Biologically Inspired assemblies like these have for many years represented an ultimate E O X u) , , h z , r. r 1,. , , I, ,
Engineering goal in protein design. By exploiting principles of symmetry that (D > c" U) Ullll/Qllgllll'l/ i; l/lll'llUL/Y

are shared by nearly all natural selfassembling structures, we have : 0 U) r , [T T Fr
In this lecture, I will describe the fundamental role that developed methods for engineering novel proteins that assemble E e a LU l.lellllOJlllllJllL LVN! [llllllllllU[llloo
mechanical forces play in control of cell and tissue development, to form a variety of complex, symmetric architectures. Recent a G) C D: 1,,
as well as how this knowledge is being leveraged to engineer successful designs include hollow protein cages composed of Q 2 '>_< D l
new bioinspired materials and devices. Living cells form and 12 or 24 identical subunits in cubic arrangements. Symmetric (1) C (D D l
function as dynamic hierarchical assemblies of nanometer scale materials that extend by growth in two or three dimensions are D D l < l
components, yet they exhibit great robustness, mechanical also possible. Natural and engineered protein assemblies will be  . .l
strength and biochemical efficiency. This is possible because discussed, along with their future prospects for synthetic biology a
they use tensegrity' architecture to mechanically stabilize their and biomedical applications, I. J

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