xt7bg7373k6b https://exploreuk.uky.edu/dips/xt7bg7373k6b/data/mets.xml University of Kentucky University of Kentucky Chemistry Department 19790423 A brochure for the Naff Symposium, an event hosted by the University of Kentucky Chemistry Department supported by the Anna S. Naff Endowment Fund. This brochure belongs to the University of Kentucky Chemistry Department Records collection, accession number 2014ua075. archival material  English University of Kentucky Chemistry Department Contact the Special Collections Research Center for information regarding rights and use of this collection. University of Kentucky Chemistry Department Naff Symposium brochures Fifth Annual Symposium on Chemistry and Molecular Biology: "Interrupted Genes and RNA Splicing" text Fifth Annual Symposium on Chemistry and Molecular Biology: "Interrupted Genes and RNA Splicing" 1979 2017 true xt7bg7373k6b section xt7bg7373k6b r— c U
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3 (i) a Fifth Annual Symposmm on
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8:30 Coffee—CF Room 137 o ‘éi- 3
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9:00 Welcome and Introduction—CF Room 139 5 9h '*
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9:15 STRUCTURE AND EXPRESSION OF INTERVENING SE- 8 g 0
QUENCESINEUCARYOTIC GENOMES «on a $- CHEMISTRY
—Dr. Beniamin Lewin— 0~ (,2— 3
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The recent discovery of interruptions in eucaryotic genes has Q and
made it necessary to discard the concept of the gene as a
contiguous length of DNA that is colinear with its product.
Intervening sequences have been characterized in nuclear MOLECULAR
and organelle genes of many eucaryotic species; their
locations in related genes may show their origin to be distant BIOLOGY
in evolution. The intervening sequences are transcribed but
are spliced out of the primary transcript to produce a
messenger RNA whose sequence is colinear with protein. In
instances in which several intervening sequences must be established in memory of
removed, discrete intermediate precursors may be found. This '
provides a new mechanism for the processing of giant nuclear ANNA S~ NAFF
RNA. Alternative pathways of RNA splicing exist in some viral
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systems, so that overlapping products are generated from a
single length of DNA. Transposition of sequences in cellular
DNA has been found in certain situations. The genetic im-
plications of this organization will be discussed.
Interrupted Genes
10:20 Discussion and Coffee Break
and RNA Splicing
10:40 ROLE OF RNA PROCESSING IN THE SYNTHESIS OF
mRNAs
—Dr. Phillip A. Sharp—
The structure of mRNAs of viruses of animal cells has shown
that RNA processing plays a critical role in the expression of
genetic information in these systems. In general, several Speakers
mRNAs are processed by RNA splicing from one transcription . ' ,
unit and these RNA species have similar sequences at both Dr‘ Benlamin Lewm
termini. The time course of synthesis and phenotypes of RNA Dr. Phillip A. Sharp
synthesis in mutant-infected cells suggests that RNA Splicing
can be regulated. These data will be discussed in terms of our
current understanding of the biochemistry of RNA splicing and
the sequence of the RNA products.
The first step in further defining the components involved in
excising intervening sequences and iaining RNA sequences 3 APRIL 23' ‘979
coding for a polypeptide is to develop an in vitro system 5 Z
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capable of carrying out this reaction. An in vttro system which -u i 70
synthesizes the correct 5’ and 3’ termini for adenovirus RNA 93 G) .C 2
has been developed. This will be discussed in terms of a gal _U l” :
hypothetical pathway for synthesis of mammalian mRNAs. ‘l .2 > 8 0
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 F'tth Annual Symposium on
Chem'str and Molecular Biology
established in memory of
Anna S. Naif
April 23, 1979
Room 139 Chemistry—Physics Building
Department of Chemistry ‘
University of Kentucky
Lexington, Kentucky 40506
Interrupted Genes
and RNA Splicing
SPEAKERS
Dr. Beniamin Lewin
Editor, Cell, MIT Press
”Structure and Expression of lntervening Sequences in Eucaryotic Genomes”
Dr. Phillip A. Sharp
Center for Cancer Research and Department of Biology
”Role of RNA Processing in the Synthesis of mRNAs"
The Symposium is supported by the Anna S. Naff Endowment Fund.
For additional information, please contact Professor Robert D. Guthrie at the Department of Chemistry; phone (606) 258—5106.
Parking will be available at Commonwealth Stadium on Cooper Drive. Shuttle buses run to the main campus.