to E i? Seventeenth Annual
1991 PROGRAM >5. 2- s
___________ 3 .0; 3 Symposmm on
ip Ln
' O 5%
AM. PM. P o :5
9:00 Registration and CoffeeRoom 137, 12:15 Buffet Lunch, Faculty Club (Please return . 7: H- O .
Chemistry-Physics Building card by April 9, 1991 for reservations. Cost 6 7: H ems ry
$6.00 to be paid at registration.) 4; g 0
9:30 Welcome by Dr. Robert Hemenway, . O Eo- :3"
Chancellor, University of Kentucky, Room 1:30 Dr. Richard J. Jackson, University of g n g
I 139, Chemistry-Physics Building Cambridge, England 0 ,5 5-
The Novel Mechanism of Initiation of b E?
9:40 Introductory RemarksDr. Robert Rhoads, Translation of Picornavirus RNAs Q \C
Department of Biochemistry, a M lecular
University 0 Kentucky The selection of the correct initiation site for translation of o
9:45 Dr. Michael B. Mathews, Cold Spring Harbor mfessenger ENA isha k'eybstep, crucial for accriiracy and efficiency .
Laboratory, New York 0 protein iosynt eSis y ribosomes. l-or t e vast rnaiority o 7'
Viruses, Interferon, RNA and the Control of eulgaryotfic C8211) mRNZisdand r30 Vlral WRNAS 1:15 appeahrs imc  M '0 0 9y
Protein Synthesis. to e ac ieve y an en - epen ent scanning mec anism, t e +L.
ribosome binds first at the end of the mRNA and then scans the (5
The initiation of protein synthesis can be controlled by of the sequence until it finds the first AUG trinucleotide codon which I \JYQIC/l'l
phosphorylation of one the initiation factors. elF-2. that catalyses is used for the initiation site. Picornaviruses (eg, poliovirus,
an early step in the pathway. Cells make use of this mechanism common cold virus, etc.) represent an intriguing departure from g Q C K80J
to regulate the translation of their own mRNA. In the presence this normal mechanism: a ~450 nucleotide segment of the viral / " I.
of interferon, they also exploit this mechanism to establish an anti RNA causes ribosomes to bind directly to a site situated just m1G WAUGANVV
viral state which prevents virus multiplication and limits the spread downstream of this region, without scanning from the end of the WWCVSS
of infection. Several viruses have elaborated countermeasures to RNA.
protect themselves against cellular defenses, These counter-
measures include inhibitors of the protein kinase .that 2:40 Discussmn .  established in the memory Of 
phosphorylates elF-2. Both activation and inhibition of the kinase _ . .
are regulated by viral RNA molecules, presenting interesting 2250 Dr: Robert E' Thach, Washington Universrty, Anna S Naff :
problems in RNA-protein recognition St' Louis
I Mechanisms of Regulation of Individual _ . t
10:45 Discussion mRNA Translation Rates
10:50 Dr. Hans Trachsel, University of Bern, Various mechanisms which allow individual mRNAs to be se gr/Cl QR". INITIATION OF
Switzerland. lectively translated at rates different from the average will be ;c\
911:4, Mediators 0f mRNA Binding to considered. The regulation of ferritin mRNA translation will be Q) \) \DEO PROTEIN SYNTHESIS
Ribosomes discussed in detail, Three or more elements regulate ferritin mRNA  1
I . . _ . . translation in response to the iron supply. The first element iden- LON-m QR %