xt7stq5rbw9w https://nyx.uky.edu/dips/xt7stq5rbw9w/data/mets.xml University of Kentucky University of Kentucky Chemistry Department 20150306 A brochure for the Naff Symposium, an event hosted by the University of Kentucky Chemistry Department supported by the Anna S. Naff Endowment Fund. This brochure belongs to the University of Kentucky Chemistry Department Records collection, accession number 2014ua075. archival material  English University of Kentucky Chemistry Department Contact the Special Collections Research Center for information regarding rights and use of this collection. University of Kentucky Chemistry Department Naff Symposium brochures Naff Symposium 2015: "Single Molecule Approaches to Deciphering Molecular Interactions in Biology" text Naff Symposium 2015: "Single Molecule Approaches to Deciphering Molecular Interactions in Biology" 2015 2017 true xt7stq5rbw9w section xt7stq5rbw9w l
E Q
._ g 3: Ni: c; i , 1 , wig, - - - - - H 8 % § cT; :
h, I 1‘ .- 1‘ it at J} M ii. J; b lab IS focused on pushing the limitsof Single-molecule E g g Q C. E If
g» : ' 1i i l l 2/ \_;:'/ :71 : Ll detection methods to study biological systems With 3 g [3 <7 .3); l
multiple components to better mimic the cellular 2 $05 a .5 .4: f @
conditions. in the first part, Iwill describe the surprising O 3' it E .3
and deep insights, revealed by multi—color FRET and CL l a;
fluorescence—force spectroscopy, on the dynamics { '
of DNA repair proteins on single stranded DNA. In the l @
8:00 A.M. second part, I will describe a single-molecule pull—down
REGISTRATION Er CONTINENTAL BREAKFAST (SiMPull) assay that combines the principles of a Ii @
Gallery, W.T, Young Library conventional pull—down assay with single—molecule i
fluorescence microscopy and enables direct Z ©
visualization of individual cellular protein complexes. 3
8245 A.M. WELCOME SiMPuIl can reveal how many proteins and of which kinds Q9
Dr. Eli Capilouto, University of Kentucky President are present in the in vivo complex and is widely applicable .‘ /
Auditorium, W.T. Young Library to various signalling proteins found in the cytosol, 3 @
membrane and cellular organelles, and to endogenous i
9 O O A M protein complexes from animal tissue extracts. :5 e @
1 . . DR. PHILLIP TINNEFELD l
1
DNA Origami Nanopnotonics: from Superresolution to . 3
Functional Devices 11'30 A‘M' LUNCH l &
In recent years, DNA nanotechnology has matured to 1:30 p.M_ POSTER SESSION i Qb¢
enable robust production of complex nanostructures M lt' R 8108C WT Y L'b g o 0
. and hybrid materials. We have combined DNA U ipurpose oom, ’ ‘ ‘ oung ' rary ;: Q. s
nanotechnology with sensitive optical detection to v Q”
create functional single-molecule devices such as 2:30 '3’”. DR. CARLOS BUSTAMANTE 8 g . ¢ «0
nagoscopic Trulersffor superlrresoDliion mlchSCOpy I Division of Labor and Coordination Among the Subunits 5 i 08
an energy rans er swr c es. origamis are a so rf - ~ - I,
used to construct nanoscale force balances with Of 0 Nearly Pe ect BIOIOQICOI Machine . m LU yo
FRET readout or for single—molecule placement in As part of their infection cycle, many viruses must >. a; 8 3 i o ,
zelrfomodebvvccijveguides :5th FGhQOdOWeFS-l Especially, package their newly replicated genomes inside a a < > O 8 , é
:znigzism:lazieizzxe'esnii‘isstrategists“ . 'g m x i, a .. 0°
for single-molecule detection at higher concentrations {3'6 mm long double-stranded DNA usrng a pentameric 0) 8 g 0 “J 1‘ 4
for biomolecular assays but also for diagnostic ring nano motor that belongs T9 the ASCE (Additional -C "" 4" Ln 0 t «'
lications i will discuss recent advancement in Strand, Consen/ed E)_superfami|y of ATPases. A number U 3 5 O S ; $.
flofoeescence enhancement and how to dis nt n le of fundamental questions remain as to the coordination q_ E X V D: .
the complex factors that generally infiuenceethce] g of the various subunits in these multimeric rings: The portal 0 __ u_ >- LLI ;
fluorescence of single molecules near metallic motor In bacteriophage phi29 IS ideal to investigate E _l O X (1)
these questions and IS a remarkable machine that must a) _: > C“ U)
nanostructures. overcome entropic, electrostatic, and DNA bending E “'5' '4: O U)
energies to package its genome to near-crystalline density 4.. o 2 ‘6'.) Lu .
10:00 A.M. BREAK AND REFRESHMENTS inSIde the capstd. Using optical tweezers, we find that this a U) a; C DC
motor can work against loads of up to ~55 picoNewtons on Q 0 .2 -; 0
average, making it one of the strongest molecular motors G) O C a) D . -
é‘e’fimii’tstie-nzigeTSS'étoi:25mm Sirisizioi‘iigzagiiiiisgzizzistgiz3842:1232? O i“ 3 4 “ ‘ SINGLE noracurmpnomuas
Eavesdropping on Single Molecular Conversations as the prohead fills, indicating that an internal pressure “E ,0 TO DECIPH ERING MOLECU LAR
builds up due to DNA compression attaining the value of ~6 - o INTERACTIONS IN BIOLOGY
Single—molecule detection has opened vast avenues MegaPascals 0i the end Of the packaging. This pressure, EU ,
to investigate aspects of biological systems that are we Show is used 05 F30” Of the mechanism 0f DNA injection ° EB ‘
inaccessible by any other technique. Research in my in the next infection cycle. Ez i
:E , CHEM.AS.UKY.EDU/NAFF-SYMPOSIUM

 DR. PHILIP TINNEFELD ,
INSTITUTE OF PHYSICAL a THEORETICAL CHEMISTRY.
BRAUNSCHWEIG UNIVERSITY OF TECH NOLOGY
, fi "an: .1 Philip Tinnefeld studied chemistry in MUnster, Montpellier (France), and Heidelberg (Germany).
: ' ' g; In 2002, he received his Ph.D. from the University of Heidelberg under Prof. J. Wolfrum.
\-_ M’h He carried out postdoctoral research with S. Weiss (UCLA), F. C. de Schryver (KU Leuven,
“v—~—— — = d" » ,3 >~~-Belgium)'and~M:»Sauer‘(Bieiefeld)-'on the-development efrsingle motecuie'fluorescence: ~~= {~— -=: -.- 3—74
l 1 5:. ,1." After habilitation in physics at Bielefeld University and three years as associate professor
i 7 53‘ of biophysics at Ludwig-Maximilians-Universitat Munich, he was appointed full professor of
_; .: ,7 biophysical chemistry at Braunschweig University of Technology in 2010.
His research is inspired by our emerging abilities to study and build matter bottom-up, starting from single molecules. He
has contributed to the breakthroughs of single-molecule superresolution microscopy by discovering the switchability
of commercially available fluorescent dyes such as Cy5 and by discovering the reducing and oxidizing system (ROXS)
which nowadays constitutes the photophysical model of antifading formulas for fluorescent dyes. He then was involved ’
in the development of three superresolution techniques including dSTORM, Blink Microscopy and DNA PAiNT. In recent
innovations, he combined optical single-molecule detection with DNA nanotechnology for self-assembled molecular f”
devices with optical readouts including energy transfer switches, calibration nanorulers, nano-adapters, optical signal
amplifiers and molecular force balances. Dr. Tinnefeld has authored more than i 10 publications and patents. He /
has received several awards including a Schloessmann Award of the Max-PIanck-Society, the Chemistry prize of the /
\ Gottlngen Academy of Science and is recipient of an ERC starting grant. /
W.Wmmmf
= DR. TAE KJIP HA
: is CO-DIRECTOR OF THE CENTER FOR THE PHYSICS OF LIVING CELLS.
-—. _ ‘ UNIVERSITY OF ILLINOIS
’ :I’ 133.. Taekjip Ha earned a doctorate in physics from the University of California, Berkeley. Prior to
" ‘77 joining the University of iliinois in 2000, he was a postdoctoral fellow at Lawrence Berkeley
' National Laboratory and a postdoctoral research associate in Steven Chu‘s laboratory in the
Department of Physics at Stanford University. Ha is a Gutgseil Professor of Physics and Biophysics
I and is an investigator with the Howard Hughes Medical Institute. He is a co-director of the
M V . ““0 -‘. 0" ICU-“MU. '- ‘0!I.i"'"" '
as the Cellular Decision Making in Cancer theme leader at the Institute for Genomic Biology
_ and as the director of PhD. program in Biophysics and Quantitative Biology.
, Dr. Ho‘s research is focused on pushing the limits of single-molecule detection methods to study complex biological
' systems. His group develops state-of-the-art biophysical techniques (e.g., multicolor fluorescence, super—resolution
imaging, combined force and fluorescence spectroscopy. vesicular encapsulation, single-molecule pull-down)
and applies them to study diverse protein—nucleic acid and protein-protein complexes, and mechanical
perturbation and response of these systems both in vitro and in vivo. Ha is the author of more than 180 papers
and has received numerous awards including Outstanding Young Researcher Award, AKPA; Research innovation
' Award, Research Corporation; Searle Scholars Award; NSF CAREER Award; Fluorescence Young Investigator Award, “-
Biophysical Society; Cottrell Scholar, Research Corporation; Beckman Fellow at Center for Advanced Studies,
_ University of Illinois; Xerox Faculty Research Award, University of Illinois; Alfred P. Sloan Fellow; Beckman Fellow,
Center for Advanced Study (CA3); Howard Hughes Medical Institute Investigator; Fellow, American Physical
Society; Michael and Kate Barany Award for Young Investigators, Biophysical Society; University Scholar, University of
Illinois; and Ho-Am Prize in Science, Ho-Am Foundation, Korea.
WWWWWWWWm.WWW.....W.Wmm...........u.m.,...............m,.... .-.........- . mm... .. W...,......
i . .
( ...- DR. CARLOS BUSTAMANTE
—- \ I
‘ : ‘3 ‘ -::3..: RAYMOND 6 BEVERLY SACRLER CHAIR OF BIOPHYSICS.
i i“ (L :5. i: J ‘ ' UNIVERSITY OF CALIFORNIA. BERKELEY
‘ l ‘ (”'51) I Carlos Bustamante uses novel methods of single-molecule visualization, such as scanning I
(j .1 ‘ L . . force microscopy, to study the structure and function of nucleoprotein assemblies. His ,
i .\ I "' laboratory is developing methods of single—molecule manipulation, such as optical and
7 .. :7 _ :gl : 7 . \. f : magnetic tweezers, to characterize the elasticity of DNA, to induce the mechanic_ql_fi___ ::__WW___ .WW--.
’ W ' V "T WW” \. ” ”"fmui'Jnfoidifi‘g of Indivraual‘b‘ro'tém anHR‘NA'Tfi‘éi'e‘cu es,'ana‘t‘6"i'fiVé‘s‘tTg-<fie'thé machine-like ‘
i behavior of molecular motors. : ‘
. Dr. Bustamante Is a professor of molecular cell biology, physics, and chemistry at the
University of California, Berkeley where he also serves as the Raymond & Beverley Sackler Chair of Biophysics. He is
; also biophysicist faculty scientist at Lawrence Berkeley National Laboratory. Dr. Bustamante is the author of more
) than 250 publications. He is a member of the National Academy of Science and was also elected as a Member of
' the Chilean Academy of Science. His accolades include the Searle Scholar award, Alfred P. Sloan Fellowship, the
Raymond and Beverly Sackler International Prize in Biophysics, the Vllcek Prize, National Science Prize of Peru, Hans
Neurath Prize of the Protein Society, and the Southern Peru Copper Corporation Prize. He has been recognized
as an Honorary Professor and Doctor Honoris Causa by the Universidad Nacional Mayor de San Marcos in Lima,
Peru; Doctor Honoris Causa by the Universidad Nacional de Ingenieria in Lima, Peru; Doctor Honoris Causa by the
Unive rsidad Peruana Cayetano in Heredia, Peru; Honorary Member of the Spanish Biophysical Society; and Doctor ,
Honoris Causa from the Universidad Peruana Ricardo Palma in Lima, Peru.
2015 Naff Committee Members
Professor Chris Richards (Chemistry, Committee Chair)
Professor Jason DeRouchey (Chemistry) 1‘ N . " F “5”“ “I .
Professor Marcelo Guzman (Chemistry) MW -
Professor Anne—Francis Miller (Chemistry) 1 6
For more information, contact Dr. Chris Richards of mm L a}
chris.richards@uky.edu ARTS&$CIENCES I 8 65 ~ 20 I S