xt7ttd9n629j https://nyx.uky.edu/dips/xt7ttd9n629j/data/mets.xml University of Kentucky University of Kentucky Chemistry Department 20090424 A brochure for the Naff Symposium, an event hosted by the University of Kentucky Chemistry Department supported by the Anna S. Naff Endowment Fund. This brochure belongs to the University of Kentucky Chemistry Department Records collection, accession number 2014ua075. archival material  English University of Kentucky Chemistry Department Contact the Special Collections Research Center for information regarding rights and use of this collection. University of Kentucky Chemistry Department Naff Symposium brochures Thirty-Fifth Annual Symposium on Chemistry and Molecular Biology: "Protein Design and Engineering" text Thirty-Fifth Annual Symposium on Chemistry and Molecular Biology: "Protein Design and Engineering" 2009 2017 true xt7ttd9n629j section xt7ttd9n629j .' ; ' ;\ 1.":- . '2 »' m .0" f .. <1 -~ . ...», ' 4 ,“ . ., m s r“ .. '. 1* 1 .- ,. ,- ' 1",st A“ ,
it 3) - {if-.11.? ..V‘i',-..’,". 13H .«4 .~ 3 .. -. 5,. , ‘ » 9".- .' ”a :s,"rx":."1.~,'1I"3.-.v‘ 2.3% : 2 xvi" t.. . M “vii“. ail" rr’av-fu ' .‘v.;‘.,-5:.".k '0 '- Fig" ‘9 r-tr' ' L: J." we." , , '1 9'.“ ‘3' x. ‘Ii'fl"? '1"'I". 1‘ w" 2 ' 333*”:- .'-~'.-' ”5:1 F“ ' fi-M' ‘- «‘"5‘ “r“ 5 '. '
my" ' ‘ r”. " ~ - '5 “m " . ,w “if“ a».- .. ‘ . .. -.-' .'.‘ ‘1"."";7, " “ ‘ I. -. ’ ‘ ' -'~_'1.~". . '5"»"‘ vim" 7' 34".» ’-. v / " ’ .’ .tt' 1’" -' 1 '~ taxi." w. ‘I I..'- ~." -' 1.”V',~-’-.‘."‘.~. ‘ .f’ 2 "i 1r ,9 _ . .6r'I_'».‘ " *‘r- r.":r ‘v ’5’"""$
1.7:: » -' i=7: ' . 5. é" , “'2."-‘.~'?t‘ ° ' ..QEQ'filt', -: ~ --...: 9'32” ... ' 5" tag-r ~.."_-‘.j'.., ', Egg-1. ,; 1 ' '. 1;, «j; .1. : .' 7" ‘7 . 75th" ° "' ‘9gyfgzr ".2 . ' . .'-.” ., 113*. 1'" j.{?""" "I i:"?‘, it 1?,
~“._,-".r-‘~~V- - , c'fij'm . f‘ : ._~. 5 3 . ‘4'».1 ”f. I“; Log. ‘1" r1 "€3.14. 1- ‘. w I ‘ 11‘5"" " -’. I ' ”45‘ E, 1,-3‘, .1 :.,_ tfll‘h. ". , ‘,- . . . 2} L, .m’“ . _ ":9." E."‘",vt!57 .1 ,-l' 1‘ ‘ . 7‘ ': :- fl; ‘ .‘ .' . If)" . 1‘. I".,-‘.,‘.-1,: 2’ ..., :p 2 “' ~‘. '“Yi'rg‘
g F .1 ~i,-.' '2 ,3} A... 1 Vt". W7 Y“ ' .9". ”€9.55 ' '2. ~ V 29' n§'1.&j ’€1%,.;; WE? ,'.'.-.. ...??? . «..9, j. w " :f’" mfg-"III (£2,132. r: ”B: ; ' . . f », 1 ..c.’ 2 ..{ii -' We» 4;?
., ,$..zj,.;g-,:, J; as" 2. _ "~ ,. ..1 ,‘ ' ”,- .' ,' ' . . _' 5.1%.: I -4 ' _. " .’_ It ,I ‘ ;3.._;,;-1‘-;‘ 'f. if". .{Ir-‘r‘y; 1:? r 1.29.”), want-",rUE‘fix’I 23‘s; Iv; 3..." .' j I, ,. 2 f . .' ”gig? 1“ . t,“ '; ~ ’ ’,"-1'.-' L. g: 3' =; .' 1- . , .5 ,',- .‘ ,- ‘ _. ' 1,. ." . .""I‘I,I, .1
. .~‘ ,' to -.-. '51 yard-:t5w5. . . ,. '29: firs" Q '-.»~='.""::i'-i‘= " » 2 13m), ‘ ’~°-'I"v.“?n'~"-” “:Y .53.,,-,-..,-*'.{-:. 1 " Y "Y " “‘4',” Y ‘9’? “if? S 7' Y‘ I ' h ' Y "{7“
, ,. 2 " , - '~' . 1 , . " ‘. . , ~"1_I ,-, '- .. ,_ , J, 4 3w “ - - _; . - ' . » . ' ‘ ‘\' \r . a": 'I ‘. . _ ~ ‘ ‘ .tt'.’ .r, ,~ ... , ' .' . 3 _ ,z .. ,- .~ :1". .V ,
—_______—________ “hWM-Mm- _. .g. , ~ 2 , » . ‘r .. gs...
.:,.’,' .;'~.. . - '“I‘
2009 PROGRAM 5.,._.,,g Thirty—Fifth Annual ;: 1
—“———————————-————————— C "x .j_-’,. ' ,, ".:',K;_«.7 . ." ‘.'. ._f ' .34 -
__—_————__—‘ .2 s ~ Symposmm on .
8:30 am. Registration & Continental Breakfast, tively, assembling into structures containing parallel 3 a) :5.— . " :21...
Room 137 Chemist -Ph ' B 'Id' d ‘— II I h r ' ' -h I' I I t - C °’ C ‘0. .
, ry ysrcs ur mg an anti para e eices, extensrve inter eica eecro as) a £2 0 . . ,1. . . , .'e"--:;' . ;
static interactions, and a solvent-excluded hydrophobic 5 8 - _Z r.-.-. 1,5 , Chem Istry ‘ 1?? 553.3% 11","):
9'00 a.m. £33339 axigrr'sr“"Ltf’lieRfitSUEbas‘Aéamy’ core. Ongoing efforts to elaborate beta-peptide bun— E 3'). :3 E E tré ...». 1'2}
139 Chemist -Phtysics BuiIst: y 00'" dles with enzyme-like metal-binding and catalysis sites DE: 3' gig: “‘ gig." & : :L'L.:_,,.j.-fl.¢7 2';
. ’Y Y 9 will also be described. e a , J ’- .. ..ri'itféii
9:10 am. Introductory Remarks - Dr. Steven W. _ 2 _' L; M I I LY
Yates, Chairman, Department of Chemis- 12:00 pm- Poster Sesswn. Rose S_treet Concourse, :55; .148: . -. O ecu ar :gf_.:fi’jja};ln
try, University of Kentucky Chemistry-PhySIcs Budding ".I..;' " '-:-r'. _‘ ' _ an”? '35,}
. . . . diam; B I "- ii" L'
9:20 a.m. Dr. Michael H. Hecht, Princeton UniverSIty l—unch. Conferen_ce_Room (GP-137). Chem- ,5: IO 09y _.
"Towards Synthetic Biology: Functional IStFY'PhYSICS BUIIde .’ #1,: ' < -'.-,;' ff"
De Novo Proteins From a Designed Artifi- _ . 3 J .
ciaIProteome" 1.30 pm. Dr. 'Peter G. Schultz, Scripps Research 3 it 93.“ \ 1,
Institute » ' ,1, ' . g "'-::; "._ [£355
. . . . " ' ' ‘“ l " 15“» ii
Synthetic biology aims to desrgn and construct afignécgfégyét [Encemlnsteggcgezg ghffg‘gg ° '99. mi / /' '3';.‘~...-r.I...'_-.".f“*i
biological systems from defined components. Initial ”en-C Code". .39"; 7:91.: 3/ V “1““?
efforts in this field typically rely on ‘toolkits’ of genes . , /’ , . 43.3 '
and proteins borrowed from pre—existing organisms, Our research program combines the tools and ‘ l . V‘
and recombined into novel arrangements. In contrast, a - . ~ - " :1 ~. Life;
truly synthetic biology would incorporate novel macro- p rinCIples .O-f chemistry with the molecules and proc— 759.,‘1'1‘ 1‘“: 4...}?
molecules designed ‘from scratch’ and synthesized in esses Of “V'ng cells to synthesrze new 'T‘O'eCU'eS and '53. i 13 3}?
the laboratory. As an initial step toward this goal, we modletcullar .aSISSmblies Wlth novel. physrcal, chemical ,.1’ established by M Benton Naff ~“
have constructed a collection of de novo roteins (an an IQ ogica unctions. By studying the structure and by, . - ..
artificial . roteome’) com risin millions 8f desi ned function of the resulting molecules, new insights can be "“535? In memory 0f Anna 3- Naff E» :lféfitegw
roteins PI'his resentatian will9 demonstrate that9 ro- gained into the mechanisms 0f complex biological and 1%? a"? €34.”
p. ' . p . . . p chemical systems. Examples of this synergistic chemi- $49.29.,” ,.-'i.1‘?§:r";;3.:tifs§iiz
terns from this artificral proteome fold into well-ordered . - - - - '- t 2' ———————— . at”
structures and e rform a ran e of biochemical and bio- cal/biological approach to synthesrs Will be discussed ~ .3; V.‘_'._-j,,‘,;3.§f' . . 5.3.7.35" agar}
Io ical function: 9 including the addition of amino acids with novel biologi— ijfirQ'..o' "‘ ”V Pratem Desrgn and ‘{ {if-
9 ' cal, chemical and physical properties to the genetic L0 . )7 En9meenn9 3
_ codes of prokaryotic and eukaryotic organisms, and the >, L0 '5}, " . 1' ———“~——*— »,r"5
10-30 3-m- Break (Refreshments AV3"ab'e) identification of small molecules that control stem cell *3 >8 iii: .,§, 2.1%.}:
. . . . $731.9:- . #1., W44.“ ,,,~v,
10:50 am. Dr. Alanna Schepartz, Yale University self-renewal. and directed differentiation, as well as E 5 (5 H. . SPEAKERS ‘..;;'.'.:.;.-“’5
"Protein Design Without a—Amino Acids" reprogramming 0f somatic ‘39”5- g 3 8 f 'T'J C 1&32’,‘
4—: O -- ..ij '2“.,’_ t Fig}? .113: 1.3;-
- - - ' - - O C » 3-”: “ . I
The catalytic, mechanical and structural fitness of 2-40 p.m. CIOS'"9 Remarks ' Dr._ “"9!" we" Depart- ... £2 : 1 4' Michael H. Hecht iv~
proteins rely on their ability to fold into discrete secon- ment 0f Chemistry, UniverSIty 0f KentUCky g u— x “a“; “7., Alanna Schepartz f ”if
dary, tertiary and quaternary structures. Non-natural g i — Err». P t G S h It 11'7“?
polymers have the potential for equally complex struc— E r: S g‘fiéé 9 er - C u Z r4563”;
ture and sophisticated function, but the design of such 1: g *5) 25,9; 2.1»;
molecules is even more challenging than protein de— “5 > C ‘53 . . *‘
, _ O.._ "' a“. .
Sign, because there exrst no natural templates to a) C E Rf: ‘Hfia Frlday’ April 24’ 2009 atmq’rfi
mimic. This lecture will describe the design, synthesis, 0 D —' 3:255? 1i. ‘%.L,
high—resolution structure, and biophysical analysis of a ”I 1;. . 35:33 “131?:
series of helical bundle proteins composed entirely of {it"fgéi;»_~__11f{_'.{'~7?fi:1}-j"' Department Of Chem'Stry "131‘
beta—amino acids. Like natural proteins containing al~ _ O f ‘ University Of Kentucky watgutfi
pha—amino acrds, beta—peptide bundles fold coopera— ihtth/wwwchemukyedu/semmars/nafil) 75:359.;- Lexington, KY 40506-0055 as
43;.» :j'. 153'. :{r' 317,. .~ ‘ Ugfisikgxr' 7151.73?in
. . . . . .' .: - I’ 9.7,. . . "spec-1“ {t..-I Iva:
UL . 2212']. '-~ we: k ..n ”' W- '- , . VW I: ~‘"4‘ “I“
’ *2 ..., 2"” “we ““K1 .i. .‘M‘mfl “1,, ‘I‘ . 1.9.,“ ’33 We»: ~". i: W “g‘mvm é.
””54“; $fi.gu\ 2- “ . "’ ween). 6 I‘EIQ‘, twfihsIfi ‘tflm‘g‘xéivée Wt: 3.379% ,Q,;"%.’X‘v~ twda‘efli?‘30fi 3.9;.
w 1921. I, . , ” ~. 1 .2 rt":— ,. ’z.‘ «' rt”: Ir .. i '9
313,. 2’3: 2éz:" I .' "it: ‘YC‘; ”3.1“?31334 g: Y .Y“ ’79,!”ngth ‘* 11.11:; Y tfi‘q“?~ai‘éfh 835‘51 ;" @Erefi’tzifi's‘f: 5”? £1;
3.2,": t" 1:sz ...... aw“... g: 51:1». -.iftmwt’mttr I7“ .. that .31 «.§W2’:«§.§g— ”.i
. 11'” “ u 7 x '3 ‘§ - ‘ Y is 3" a» ”“33"“? ft‘”"”::“ Law" 773$ “fi' . tyt’i‘fitett it»??? 5%,, ”t5;

 ’ 1 V2” .
Department of Chemistry, Unrversrty of Kentucky ‘ ijgf' j
. presents the » . f

Thirty-Fifth Annual Symposrum on L
» ,_- g gag: I?
, Chemistry &
Molecular B'ology '
Established by M. Benton Naff In memory of Anna S. Naff , r. 7 -
v, Friday, Aprrl 24, 2009 9:00 am. g 2
Room 139, Chemrstry-Physrcs Burldrng Ly; -'
I .i' . 1'35“};
Protein Desrgn and Engineering I ,.
‘ Michael H. Hecht, Professor of Chemistry, Princeton University. Dr. Hecht was born and grew up f ff: f, .
_ g in midtown Manhattan. He received his BA. in Chemistry from Cornell University, where he did under- _' '-; I"
; mtg graduate research on protein folding with Professor Harold Scheraga. He completed his PhD. in Bioi- g
@146: ogy at MIT, where he received the first Ph.D. from Professor Bob Sauer’s lab, and did research on ~ g' ‘ ‘ ’
I}; i‘wtx' protein stability and protein/DNA interactions. He did post-doctoral research on protein design in the - l: e;
- ‘ :5; ’g’ labs of Professors David and Jane Richardson in the Biochemistry Department at Duke University g: gm;
- ' 3 Medical School. In 1990, Hecht joined the faculty of the Chemistry Department at Princeton, where he g
i" also holds an affiliated appointment In the Department of Molecular Biology. At Princeton Hecht has 3 _ _,
, served as the Director of Undergraduate Studies and Associate Chair of the Chemistry Department. f
Hecht’s honors and awards include a Whitaker Foundation Young Investigator Award, a Beckman , ‘ g
’ Young Investigator Award, and the Protein Society Kaiser Award. He serves on the Editorial Advisory L‘ " 3?
‘ Board of Protein Science, Protein Engineering Design & Selection (PEDS), and Biopolymers. Research in the Hecht T11 ‘ .
laboratory focuses in two areas: the first deals with the design of novel proteins and artificial proteomes and the second _ . , fife?
j is concerned with protein misfolding, the molecular determinants of Alzheimer’s disease, and the search for anti- ‘ .. g
‘ Alzheimer’stherapeutics. g f
L I: Alanna Schepartz, Milton Harris '29 PhD. Professor of Chemistry, Professor of Molecular, Cellu- (“g I
:1 Lg lar and Developmental Biology, Yale University. Dr. Alanna Schepartz was born in Forest Hills, "
'3 ,4 New York on January 9, 1962. From 1978-1982 she attended the State University of New York at Al— ‘ ‘ ‘ g
j: , ~.« 3%... bany where she majored in Chemistry and carried out undergraduate research on organic electro- ‘ g'
*‘t if; ,3; jg chemistry with Shelton Bank. In 1982 Alanna moved to New York City to attend graduate school at . g »
“ YE?“ Columbia University. Here she joined Ronald Breslow's laboratory and received her doctorate in 1987 -. .- ,5;
;. »~‘ ; for research on the mechanism of catalysis by the enzyme carboxypeptidase A. Alanna then traveled . _ “-gf
‘ , , to Peter Dervan's laboratory at the California Institute of Technology where she developed chemical ‘ ,
. ‘ methods to explore the topology of eukaryotic ribosomes and the interactions of ribosomal RNAs with . ‘ » Z ~. ‘.
' ‘ antibiotics. In 1988 she joined the Chemistry Department at Yale University where she is now the Mil- g _;
ton Harris ‘29 PhD. Professor of Chemistry and Professor of Molecular, Cellular, and Developmental ; '
Biology. Research in the Schepartz group focuses on the chemical biology of protein-protein and protein-DNA interac- .., j _g
tions inside the cell, defining the structural and energetic factors that distinguish specific macromolecular interfaces and g f, “15;:
how the assembly and disassembly of these complexes define biology. Alanna Schepartz has received a Presidential ' _ “xii—53:11
‘ Young investigator Award (1990), a Morse Faculty Fellowship (1990), a David and Lucile Packard Fellowship Award ; Q“
f (1991), an Eli Lilly Fellowship (1991), a Camille and Henry Dreyfus Teacher-Scholar Award (1993), an Alfred P. Sloan ‘j‘ 3' " v~
; Research Fellowship (1994), an Arthur C. Cope Scholar Award (1995), the A.C.S. Lilly Award in Biological Chemistry i; g "
: (1997), the Dylan Hixon '88 Award for Teaching Excellence in the Natural Sciences (1999), the Iota Sigma Pi Agnes Fay ;‘ - g.
-, Morgan Research Award (2002), and has been named a Professor of the Howard Hughes Medical Institute (2003). In . ~
‘ addition, she has been a member (1995-1998) and Chair (1998—2000) of the NIH. Study Section on Bioorganic and '3', ,
Natural Products Chemistry and serves on the advisory boards of several journals including the Journal of the American - {Zia ‘
Chemical Society, Chemistry & Biology, and Current Opinion in Chemical Biology. 3 _ " 71:; ‘4
:3 iift Igag '2' '7 1‘ Peter G. Schultz, Scripps Professor of Chemistry, The Scripps Research Institute. Dr. Schultz l w
. i ' ‘ ‘ I; C rig did his undergraduate and graduate work at the California Institute of Technology. His thesis work with -g - ._
{g i; i. Peter Dervan resulted in the first synthetic molecules (polypyrroleamides) that sequence—selectively 1,; .j. ,' g A
j? "j ",W cleave DNA. In 1985, after postdoctoral studies at the Massachusetts Institute of Technology with 2'? “5‘ ' . L
L » EC“ .. Chris Walsh, he joined the faculty of the University of California at Berkeley, where he was Professor of g‘ , g;
i L, 7- Chemistry, Principal Investigator at Lawrence Berkeley National Laboratory and an Investigator of the . (E;
, A? g , Howard Hughes Medical Institute. Schultz joined the faculty of Scripps in 1999 where he is currently " ' ' j,..-"-;
'. the Scripps Professor of Chemistry. He is also the Institute Director of the Genomics Institute of the ‘ j. ’9 g'
__ Novartis Research Foundation (GNF) in San Diego, CA. The 500+ staff at GNF are developing and I ’ ' ‘
. ' applying state of the art high throughput chemical, proteomics and genomics technologies to identify ,
‘ novel genes and biological processes, as well as develop new therapeutics for human disease. In I f 'g
1 addition, Schultz is a founder of Afiymax Research Institute, Syrrx, Kalypsys, Phenomix, Symyx Therapeutics, Ilypsa, Ia, '
1 Ambrx and Wildcat Technologies, pioneers in the application of diversity based approaches to problems in chemistry, 5.: ‘ 5
, materials science and medicine. His awards include the Waterman Award of the National Science Foundation, member- ..g_ ' ,_ '
ship in the National Academy of Sciences and National Institute of Medicine, the 1994 Wolf Prize in Chemistry, the 2003 f g _
Paul Ehrlich Prize, and the 2005 Arthur C. Cope Award of the American Chemical Society. ”us -
FREE PARKING is available. For information contact Melissa Wirt at (859) 257-7080 or mgwirt2@email.uky.edu. For .flgg
, additional symposium information, contact Professor Yinan Wei, Department of Chemistry, (859) 257—7085 or 3 f“
. yinan.wei@uky.edu. 55*.- 3; .-",‘;;;:it
2009 Committee: Yinan Wei (Chair), Sylvia Daunert, Anne-Frances Miller, David W. Rodgers (Biochemistry) g“; ,
Symposium supported by the Anna S. Naff Endowment Fund _ “3i
2’ I g?“ {5L .